|
KMID : 0358420070500010101
|
|
Korean Journal of Obstetrics and Gynecology
2007 Volume.50 No. 1 p.101 ~ p.110
|
|
|
Establishment and characterization of secondary drug resistant cell line originating from cisplatin-resistant SNU-8
|
|
An Chun-San
Kim Sung-Yob
|
|
|
Abstract
|
|
|
Objective: Multidrug resistance to chemotherapy is a major obstacle in attempts to improve the clinical outcome of ovarian carcinoma patients. The aim of this study is to establish secondary anticancer drug resistant cell line from original SNU-8/WT resistant to cisplatin and characterize it.
Methods: After establishing secondary drug resistant cell line (SNU-8/Fac), drug sensitivity was measured by MTT assay. Multidrug resistance (MDR) was analyzed by RT-PCR and western blotting analysis.
Results: MTT assay data demonstrated that MDR was expressed in SNU-8/Fac. In addition, mRNA expression of MDR1, ATP7B in SNU-8/Fac was increased. However, overexpression of MRP1, BCRP, TS and MT mRNA was not observed. At the protein level, protein P-gp, ATP7B were overexpressed in SNU-8/Fac.
Conclusion: We established a new anticancer drug resistant cell line from original SNU-8/WT. Overexpression of P-gp and ATP7B was observed in SNU-8/Fac.
|
|
|
KEYWORD
|
|
|
Ovarian cancer, Multidrug resistance, P-gp/MDR1, ATP7B, Cisplatin
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|